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Rat B-lymphocyte antigen (CD20) ELISA Kit

Rat B-lymphocyte antigen (CD20) ELISA Kit

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Specification

Product name Rat B-lymphocyte antigen (CD20) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat B-lymphocyte antigen (CD20) ELISA Kit employs a two-site sandwich ELISA to quantitate MS4A1 in samples. An antibody specific for MS4A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMS4A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MS4A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MS4A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MS4A1; B1; Bp35; CD20; LEU-16; MGC3969; MS4A2; S7; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor

Product Properties

Kit components • Rat B-lymphocyte antigen microplate
• Rat B-lymphocyte antigen standard
• Rat B-lymphocyte antigen detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat B-lymphocyte antigen (CD20) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MS4A1. No significant cross-reactivity or interference between Rat MS4A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background B-lymphocyte antigen (CD20) gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.
Alternative MS4A1; B1; Bp35; CD20; LEU-16; MGC3969; MS4A2; S7; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor

Image & description

Fig.1. Rat B-lymphocyte antigen (CD20) Standard Curve.

Fig.1. Rat B-lymphocyte antigen (CD20) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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