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Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit

Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit

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Specification

Product name Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit employs a two-site sandwich ELISA to quantitate CORIN in samples. An antibody specific for CORIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCORIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CORIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CORIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative CORIN; ATC2; CRN; Lrp4; MGC119742; TMPRSS10; atrial natriuteric peptide-converting enzyme; corin; corin; serine protease; heart specific serine proteinase; pro-ANP-convertase; pro-ANP-converting enzyme

Product Properties

Kit components • Rat Atrial natriuretic peptide-converting enzyme microplate
• Rat Atrial natriuretic peptide-converting enzyme standard
• Rat Atrial natriuretic peptide-converting enzyme detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CORIN. No significant cross-reactivity or interference between Rat CORIN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Corin encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase.The corin cDNA encodes a deduced 1,042-amino acid, type II transmembrane protein. At its N terminus, corin contains a short cytoplasmic domain and a transmembrane sequence. In the extracellular region of corin, there are 2 frizzled-like cysteine-rich motifs, 7 low density lipoprotein receptor repeats, a macrophage scavenger receptor-like domain, and a trypsin-like protease domain at the C terminus.
Gene ID 289596
Alternative CORIN; ATC2; CRN; Lrp4; MGC119742; TMPRSS10; atrial natriuteric peptide-converting enzyme; corin; corin; serine protease; heart specific serine proteinase; pro-ANP-convertase; pro-ANP-converting enzyme
Accession Q80YN4

Image & description

Fig.1. Rat Atrial natriuretic peptide-converting enzyme (CORIN) Standard Curve.

Fig.1. Rat Atrial natriuretic peptide-converting enzyme (CORIN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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