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Rat Arylamine N-acetyltransferase 2 (NAT2) ELISA Kit

Rat Arylamine N-acetyltransferase 2 (NAT2) ELISA Kit

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Specification

Product name Rat Arylamine N-acetyltransferase 2 (NAT2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Arylamine N-acetyltransferase 2 (NAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT2 in samples. An antibody specific for NAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NAT2; AAC2; PNAT; N-acetyltransferase 2; arylamide acetylase 2

Product Properties

Kit components • Rat Arylamine N-acetyltransferase 2 microplate
• Rat Arylamine N-acetyltransferase 2 standard
• Rat Arylamine N-acetyltransferase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Arylamine N-acetyltransferase 2 (NAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NAT2. No significant cross-reactivity or interference between Rat NAT2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NAT2 isa type of N-acetyltransferase. The NAT2 isozyme functions to both activate and deactivate arylamine and hydrazine drugs and carcinogens. Polymorphisms in this gene are responsible for the N-acetylation polymorphism in which human populations segregate into rapid, intermediate, and slow acetylator phenotypes. Polymorphisms in NAT2 are also associated with higher incidences of cancer and drug toxicity. NAT2 encodes the polymorphic NAT protein because the gene product had an identical apparent molecular weight to that of NAT detected in human liver cytosol and the deduced amino acid sequence of NAT2 contained 6 peptide sequences that had previously been determined from tryptic peptides of the polymorphic NAT purified from human liver.
Gene ID 100008974
Alternative NAT2; AAC2; PNAT; N-acetyltransferase 2; arylamide acetylase 2
Accession P11246

Image & description

Fig.1. Rat Arylamine N-acetyltransferase 2 (NAT2) Standard Curve.

Fig.1. Rat Arylamine N-acetyltransferase 2 (NAT2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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