|Product name||Rat Anti-gonadotropin-releasing hormone receptor antibody (GNRHR-Ab) ELISA Kit|
|Applications notes||This Rat Anti-gonadotropin-releasing hormone receptor antibody (GNRHR-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GNRHR-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated GNRHR-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled GNRHR-Ab and unlabeled GNRHR-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GNRHR-Ab in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Anti-gonadotropin-releasing hormone receptor antibody microplate
• Rat Anti-gonadotropin-releasing hormone receptor antibody standard
• Rat Anti-gonadotropin-releasing hormone receptor antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Anti-gonadotropin-releasing hormone receptor antibody (GNRHR-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GNRHR-Ab. No significant cross-reactivity or interference between Rat GNRHR-Ab and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The gonadotropin-releasing hormone receptor (GNRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate.This receptor is a 60 kDa G protein-coupled receptor and resides primarily in the pituitary and is responsible for eliciting the actions of LHRH after its release from the hypothalamus. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers.|
Fig.1. Rat Anti-gonadotropin-releasing hormone receptor antibody (GNRHR-Ab) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.