|Product name||Rat Anti-endomysial IgA antibody (EMA-IgA) ELISA Kit|
|Applications notes||This Rat Anti-endomysial IgA antibody (EMA-IgA) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EMA-IgA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated EMA-IgA and incubated. The competitive inhibition reaction is launched between with HRP labeled EMA-IgA and unlabeled EMA-IgA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of EMA-IgA in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Anti-endomysial IgA antibody microplate
• Rat Anti-endomysial IgA antibody standard
• Rat Anti-endomysial IgA antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Anti-endomysial IgA antibody (EMA-IgA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat EMA-IgA. No significant cross-reactivity or interference between Rat EMA-IgA and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The presence of anti-endomysial (EMA) IgA antibodies has been shown to correlate with gluten-sensitive enteropathy such as celiac disease (CD) and dermatitis herpetiformis (DH). EMA is detected primarily by IFA, using monkey esophagus as a substrate and observing fluorescence of the endomysial lining. Patients with CD and DH can also demonstrate antibodies to reticulin and gliadin, though EMA-IgA seems to be the most specific marker (specifically 94-100%).|
Fig.1. Rat Anti-endomysial IgA antibody (EMA-IgA) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.