|Product name||Rat Anti-alpha-Fodrin IgG/IgA (A-Fodrin-IgG/IgA) ELISA Kit|
|Applications notes||This Rat Anti-alpha-Fodrin IgG/IgA (A-Fodrin-IgG/IgA) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A-Fodrin-IgG/IgA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated A-Fodrin-IgG/IgA and incubated. The competitive inhibition reaction is launched between with HRP labeled A-Fodrin-IgG/IgA and unlabeled A-Fodrin-IgG/IgA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of A-Fodrin-IgG/IgA in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Anti-alpha-Fodrin IgG/IgA microplate
• Rat Anti-alpha-Fodrin IgG/IgA standard
• Rat Anti-alpha-Fodrin IgG/IgA detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Anti-alpha-Fodrin IgG/IgA (A-Fodrin-IgG/IgA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat A-Fodrin-IgG/IgA. No significant cross-reactivity or interference between Rat A-Fodrin-IgG/IgA and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits. Fodrin is important for maintaining normal membrane structure and supporting cell surface protein function. Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products. Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.|
Fig.1. Rat Anti-alpha-Fodrin IgG/IgA (A-Fodrin-IgG/IgA) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.