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Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit

Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit

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Specification

Product name Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate AT1R-Ab in samples. An antibody specific for AT1R-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAT1R-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AT1R-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AT1R-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative AT1R-Ab; AGTR1; AG2S; AG2-S; AGTR1A; AGTR1B; AT1; AT1B; AT2R1; AT2-R1; AT2R1A; AT2R1B; HAT1R; AT1 Receptor; Angiotensin Receptor 1B

Product Properties

Kit components • Rat Angiotensin II receptor type 1 antibody microplate
• Rat Angiotensin II receptor type 1 antibody standard
• Rat Angiotensin II receptor type 1 antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Rat AT1R-Ab. No significant cross-reactivity or interference between Rat AT1R-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Angiotensin II receptor, type 1 or AT1 receptor is an angiotensin receptor. It has vasopressor effects and regulates aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system.It is the best elucidated angiotensin receptor.The angiotensin receptor is activated by the vasoconstricting peptide angiotensin II. The activated receptor in turn couples to Gq/11 and thus activates phospholipase C and increases the cytosolic Ca2+ concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C. Activated receptor also inhibits adenylate cyclase and activates various tyrosine kinases.
Alternative AT1R-Ab; AGTR1; AG2S; AG2-S; AGTR1A; AGTR1B; AT1; AT1B; AT2R1; AT2-R1; AT2R1A; AT2R1B; HAT1R; AT1 Receptor; Angiotensin Receptor 1B

Image & description

Fig.1. Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) Standard Curve.

Fig.1. Rat Angiotensin II receptor type 1 antibody (AT1R-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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