|Product name||Rat Androgen ELISA Kit|
|Applications notes||This Rat Androgen ELISA Kit employs a two-site sandwich ELISA to quantitate Androgen in samples. An antibody specific for Androgen has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAndrogen present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Androgen is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Androgen bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Androgen ELISA Kit microplate
• Rat Androgen ELISA Kit standard
• Rat Androgen ELISA Kit detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Androgen ELISA Kit has high sensitivity and excellent specificity for detection of Rat Androgen. No significant cross-reactivity or interference between Rat Androgen and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||An androgen (from Greek andro meaning male human being) is any natural or synthetic compound, usually a steroid hormone, that stimulates or controls the development and maintenance of male characteristics in vertebrates by binding to androgen receptors.This includes the activity of the primary male sex organs and development of male secondary sex characteristics. Androgens were first discovered in 1936. Androgens increase in both boys and girls during puberty.Androgens are also the original anabolic steroids and the precursor of all estrogens. The primary and most well-known androgen is testosterone.Dihydrotestosterone (DHT) and androstenedione are less known generally, but are of equal importance in male development.DHT in the embryo life causes differentiation of penis, scrotum and prostate. Later in life DHT contributes to balding, prostate growth and sebaceous gland activity. Although androgens are described as male sex hormones, both males and females have them to varying degrees, as is also true of estrogens. They are one of three types of sex hormones, the others being estrogens like estradiol and progestogens like progesterone.|
Fig.1. Rat Androgen Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.