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Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit

Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit

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Specification

Product name Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFRA in samples. An antibody specific for PDGFRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDGFRA; CD140A; MGC74795; PDGFR2; RHEPDGFRA; CD140 antigen-like family member A; PDGF-R-alpha; PDGFRA/BCR fusion; platelet-derived growth factor receptor alpha; rearranged-in-hypereosinophilia-platelet derived

Product Properties

Kit components • Rat Alpha-type platelet-derived growth factor receptor microplate
• Rat Alpha-type platelet-derived growth factor receptor standard
• Rat Alpha-type platelet-derived growth factor receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDGFRA. No significant cross-reactivity or interference between Rat PDGFRA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Alpha-type platelet-derived growth factor receptor is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Gene ID 25267
Alternative PDGFRA; CD140A; MGC74795; PDGFR2; RHEPDGFRA; CD140 antigen-like family member A; PDGF-R-alpha; PDGFRA/BCR fusion; platelet-derived growth factor receptor alpha; rearranged-in-hypereosinophilia-platelet derived
Accession P20786

Image & description

Fig.1. Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) Standard Curve.

Fig.1. Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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