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Rat Alpha- (1,3)-fucosyltransferase (FUT4) ELISA Kit

Rat Alpha- (1,3)-fucosyltransferase (FUT4) ELISA Kit

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Specification

Product name Rat Alpha- (1,3)-fucosyltransferase (FUT4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Alpha- (1,3)-fucosyltransferase (FUT4) ELISA Kit employs a two-site sandwich ELISA to quantitate FUT4 in samples. An antibody specific for FUT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFUT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FUT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FUT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative FUT4; CD15; ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1; ELAM ligand fucosyltransferase; Lewis X; fucosyltransferase 4; fucosyltransferase IV; galactoside 3-L-fucosyltransferase; stage-specific embryonic antigen

Product Properties

Kit components • Rat Alpha- microplate
• Rat Alpha- standard
• Rat Alpha- detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Alpha- (1,3)-fucosyltransferase (FUT4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat FUT4. No significant cross-reactivity or interference between Rat FUT4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The sialyl Lewis x oligosaccharide determinant is an essential component of leukocyte counterreceptors for E-selectin and P-selectin mediated adhesions of leukocytes. This oligosaccharide molecule is displayed on the surfaces of granulocytes, monocytes, and natural killer cells. Formation of leukocyte adhesions to these selectins is an early and important step in the process that ultimately allows leukocytes to leave the vascular tree and become recruited into lymphoid tissues and sites of inflammation. FUT7 was expressed in HL-60 cells, a human promyelocytic cell line, and in YT cells, a natural killer-like cell line. Sequence comparisons indicate that the predicted polypeptide sequence shares approximately 38 to 47% overall identity with other FUTs.
Alternative FUT4; CD15; ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1; ELAM ligand fucosyltransferase; Lewis X; fucosyltransferase 4; fucosyltransferase IV; galactoside 3-L-fucosyltransferase; stage-specific embryonic antigen
Accession Q62994

Image & description

Fig.1. Rat Alpha- (1,3)-fucosyltransferase (FUT4) Standard Curve.

Fig.1. Rat Alpha- (1,3)-fucosyltransferase (FUT4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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