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Rat Alpha-1-antitrypsin (SERPINA1) ELISA Kit

Rat Alpha-1-antitrypsin (SERPINA1) ELISA Kit

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Specification

Product name Rat Alpha-1-antitrypsin (SERPINA1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Alpha-1-antitrypsin (SERPINA1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA1 in samples. An antibody specific for SERPINA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINA1; A1A; A1-AT; AAT; MGC23330; MGC9222; PI; PI1; PRO2275; OTTHUMP00000197150; protease inhibitor 1 (anti-elastase); alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor; clade A; member 1; serine; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1

Product Properties

Kit components • Rat Alpha-1-antitrypsin microplate
• Rat Alpha-1-antitrypsin standard
• Rat Alpha-1-antitrypsin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Alpha-1-antitrypsin (SERPINA1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINA1. No significant cross-reactivity or interference between Rat SERPINA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background alpha1-antitrypsin, an acute phase protein, is the prototypic member of the serpin super family and a major inhibitor of serine proteases. As an acute phase protein, alpha 1-antitrypsin is thought to play an important role in limiting host tissue injury at sites of inflammation. A1AT is a 52-kDa serpin (serine protease inhibitor), and, in medicine, it is considered the most prominent serpin; the terms alpha 1-antitrypsin and protease inhibitor (Pi) are often used interchangeably.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).
Gene ID 24648
Alternative SERPINA1; A1A; A1-AT; AAT; MGC23330; MGC9222; PI; PI1; PRO2275; OTTHUMP00000197150; protease inhibitor 1 (anti-elastase); alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor; clade A; member 1; serine; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1
Accession P17475

Image & description

Fig.1. Rat Alpha-1-antitrypsin (SERPINA1) Standard Curve.

Fig.1. Rat Alpha-1-antitrypsin (SERPINA1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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