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Rat Adrenomedullin 2 (ADM2) ELISA Kit

Rat Adrenomedullin 2 (ADM2) ELISA Kit

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Specification

Product name Rat Adrenomedullin 2 (ADM2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Adrenomedullin 2 (ADM2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM2 in samples. An antibody specific for ADM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADM2; RP4-579N16.10; AM2; FLJ21135; dJ579N16.4; OTTHUMP00000196596; intermedin

Product Properties

Kit components • Rat Adrenomedullin 2 microplate
• Rat Adrenomedullin 2 standard
• Rat Adrenomedullin 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Adrenomedullin 2 (ADM2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADM2. No significant cross-reactivity or interference between Rat ADM2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADM2 belongs to a family of calcitonin related peptide hormones important for regulating diverse physiologic functions and the chemical composition of fluids and tissues. ADM2 contains a dibasic cleavage site, followed by a 47-amino acid mature peptide hormone sequence and an alpha-amidation donor glycine at the C terminus. Immunohistochemical analysis of several mouse tissues detected Adm2 in pituitary and stomach. PCR analysis of a human gastrointestinal panel detected ADM2 in almost all regions examined. Immunohistochemical staining showed ADM2 in the muscularis mucosae layer of stomach. Western blot analysis of transfected embryonic kidney cells found mature ADM2 secreted into the culture medium; the apparent molecular mass was about 5.0 kD.
Gene ID 399475
Alternative ADM2; RP4-579N16.10; AM2; FLJ21135; dJ579N16.4; OTTHUMP00000196596; intermedin
Accession P61312

Image & description

Fig.1. Rat Adrenomedullin 2 (ADM2) Standard Curve.

Fig.1. Rat Adrenomedullin 2 (ADM2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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