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Rat Adenosine receptor A3 (ADORA3) ELISA Kit

Rat Adenosine receptor A3 (ADORA3) ELISA Kit

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Specification

Product name Rat Adenosine receptor A3 (ADORA3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Adenosine receptor A3 (ADORA3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA3 in samples. An antibody specific for ADORA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADORA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADORA3; RP11-552M11.7; A3AR; AD026; bA552M11.5;

Product Properties

Kit components • Rat Adenosine receptor A3 microplate
• Rat Adenosine receptor A3 standard
• Rat Adenosine receptor A3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Adenosine receptor A3 (ADORA3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADORA3. No significant cross-reactivity or interference between Rat ADORA3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The receptor is an adenosine receptor, but also denotes the human gene encoding it.Adenosine A3 receptors are G protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. It mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death.An adenosine A3 receptor agonist (CF-101) is in clinical trials for the treatment of rheumatoid arthritis. In a mouse model of infarction the A3 selective agonist CP-532,903 protected against myocardial ischemia and reperfusion injury.
Alternative ADORA3; RP11-552M11.7; A3AR; AD026; bA552M11.5;
Accession O02667

Image & description

Fig.1. Rat Adenosine receptor A3 (ADORA3) Standard Curve.

Fig.1. Rat Adenosine receptor A3 (ADORA3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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