Login Register
English
0

Cart

$ 0

Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit

Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit

Views(184) Publications(0) Catalog no(KTE100287)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit employs a two-site sandwich ELISA to quantitate SCD1 in samples. An antibody specific for SCD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SCD1

Product Properties

Kit components • Rat Acyl-CoA desaturase 1 microplate
• Rat Acyl-CoA desaturase 1 standard
• Rat Acyl-CoA desaturase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SCD1. No significant cross-reactivity or interference between Rat SCD1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates .
Alternative SCD1

Image & description

Fig.1. Rat Acyl-CoA desaturase 1 (Scd1/Scd) Standard Curve.

Fig.1. Rat Acyl-CoA desaturase 1 (Scd1/Scd) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Acyl-CoA desaturase 1 (Scd1/Scd) ELISA Kit”