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Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) ELISA Kit

Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) ELISA Kit

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Specification

Product name Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS7 in samples. An antibody specific for ADAMTS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADAMTS7; ADAM-TS7; DKFZp434H204; COMPase; a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif

Product Properties

Kit components • Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 microplate
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 standard
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADAMTS7. No significant cross-reactivity or interference between Rat ADAMTS7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADAMTS7 is a member of the ADAM protein family . The gene is expressed in human brain, heart, lung, liver, pancreas, kidney, skeletal muscle, and placenta. The murine protein is expressed at low levels throughout mouse development. The predicted 997-amino acid ADAMTS7 protein has a domain structure characteristic of ADAMTSs. The mature ADAMTS7 protein has 2 potential N-linked glycosylation sites. Northern blot analysis detected a 5-kb ADAMTS7 transcript in all human tissues examined, namely brain, heart, lung, liver, pancreas, kidney, skeletal muscle, and placenta; skeletal muscle expressed an additional 7-kb transcript. Mouse Adamts7 was expressed at low levels throughout mouse development.
Gene ID 315879
Alternative ADAMTS7; ADAM-TS7; DKFZp434H204; COMPase; a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif
Accession Q1EHB3

Image & description

Fig.1. Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) Standard Curve.

Fig.1. Rat A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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