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Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) ELISA Kit

Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) ELISA Kit

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Specification

Product name Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS1 in samples. An antibody specific for ADAMTS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADAMTS1; C3-C5; KIAA1346; METH1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 1; human metalloproteinase with thrombospondin type 1 motifs

Product Properties

Kit components • Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 microplate
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 standard
• Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADAMTS1. No significant cross-reactivity or interference between Rat ADAMTS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADAMTS1 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.
Alternative ADAMTS1; C3-C5; KIAA1346; METH1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 1; human metalloproteinase with thrombospondin type 1 motifs
Accession Q9WUQ1

Image & description

Fig.1. Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) Standard Curve.

Fig.1. Rat A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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