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Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit

Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit

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Specification

Product name Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKL in samples. An antibody specific for PFKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PFKL; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; PFK-B; 6-phosphofructokinase; liver type; liver phosphofructokinase; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B; phosphofructoki

Product Properties

Kit components • Rat 6-phosphofructokinase, liver type microplate
• Rat 6-phosphofructokinase, liver type standard
• Rat 6-phosphofructokinase, liver type detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PFKL. No significant cross-reactivity or interference between Rat PFKL and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL), and platelet (PFKP), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, M3L, M2L2, and ML3 hybrid forms of the holoenzyme.
Gene ID 25741
Alternative PFKL; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; PFK-B; 6-phosphofructokinase; liver type; liver phosphofructokinase; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B; phosphofructoki
Accession P30835

Image & description

Fig.1. Rat 6-phosphofructokinase, liver type (PFKL) Standard Curve.

Fig.1. Rat 6-phosphofructokinase, liver type (PFKL) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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