|Product name||Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit|
|Applications notes||This Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit employs a two-site sandwich ELISA to quantitate NT5E/CD73 in samples. An antibody specific for NT5E/CD73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNT5E/CD73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NT5E/CD73 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NT5E/CD73 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat 5'-nucleotidase/cluster of differentiation 73 microplate
• Rat 5'-nucleotidase/cluster of differentiation 73 standard
• Rat 5'-nucleotidase/cluster of differentiation 73 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NT5E/CD73. No significant cross-reactivity or interference between Rat NT5E/CD73 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 220.127.116.11) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane.The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases . Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate|
Fig.1. Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.