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Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit

Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit

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Specification

Product name Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SRD5A2 in samples. An antibody specific for SRD5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRD5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SRD5A2; MGC138457; 3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2

Product Properties

Kit components • Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 microplate
• Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 standard
• Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SRD5A2. No significant cross-reactivity or interference between Rat SRD5A2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SRD5a2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). Steroid 5-alpha-reductase catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT).The cDNA contains a long 3-prime untranslated region and encodes a hydrophobic 254-amino acid protein that shares 50% sequence identity with human SRD5A1 and 46% identity with rat 5-alpha-reductase. The biochemical and pharmacologic properties of the enzyme were consistent with its being the major isozyme in genital tissue.
Gene ID 64677
Alternative SRD5A2; MGC138457; 3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid 5-alpha-reductase 2
Accession P31214

Image & description

Fig.1. Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) Standard Curve.

Fig.1. Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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