| Product name | Rat 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit |
| Reactivity | Rat |
| Applications | ELISA |
| Applications notes | This Rat 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP7B1 in samples. An antibody specific for CYP7B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP7B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7B1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | CYP7B1; CBAS3; CP7B; SPG5A; cytochrome P450; subfamily VIIB (oxysterol 7 alpha-hydroxylase); polypeptide 1; oxysterol 7alpha-hydroxylase |
| Kit components | • Rat 25-hydroxycholesterol 7-alpha-hydroxylase microplate • Rat 25-hydroxycholesterol 7-alpha-hydroxylase standard • Rat 25-hydroxycholesterol 7-alpha-hydroxylase detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Rat 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYP7B1. No significant cross-reactivity or interference between Rat CYP7B1 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CYP7B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in CYP7B1 have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. |
| Gene ID | 25429 |
| Alternative | CYP7B1; CBAS3; CP7B; SPG5A; cytochrome P450; subfamily VIIB (oxysterol 7 alpha-hydroxylase); polypeptide 1; oxysterol 7alpha-hydroxylase |
| Accession | Q63688 |
Fig.1. Rat 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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