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Rabbit IgG (Light Chain Specific) Monoclonal Antibody

Rabbit IgG (Light Chain Specific) Monoclonal Antibody

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Specification

Product name Rabbit IgG (Light Chain Specific) Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Rabbit
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa.The Light Chain Specific antibody reacts with light chain of IgG but not with the heavy chain of IgG.
Others The antibody reacts with the light chains on rabbit IgG and with those common to other rabbit immunoglobulins. The antibody does not react with the heavy chain of rabbit IgG. The antibody minimal cross-reaction with bovine, goat, Armenian hamster, horse,.
Observed Band(KD) 25

Image & description

Fig. Western blot analysis of Reduced Rabbit IgG, H+L, diluted at 1:10000.

Fig. Western blot analysis of Reduced Rabbit IgG, H+L, diluted at 1:10000.

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