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PurKine™ Protein L Packed Column

PurKine™ Protein L Packed Column

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Specification

Product name PurKine™ Protein L Packed Column
Applications notes PurKine™ Protein L Resin has been designed to purify of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein L has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein L resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Alternative Protein L; Protein L resin

Product Properties

Formulation Prepacked columns prepacked with 50% slurry in 20% ethanol.
Features & Benefits • High capacity—More than 15mg human lgG per mL of resin.
• High performance—Recombinant Protein L is produced in E.coli.It binds Igs through interactions with the light kappa chains.Protein L binds a wider range of Ig classes than Protein A or G. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Storage buffer Liquid in PBS buffer containing 20% ethanol.
Storage instructions Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PurKine™ Protein L Purification system is effective for affinity purification of mammalian IgG that contain specific kappa light chains from serum, ascites fluid, cell culture supernantant and other antibody samples. Recombinant Protein L is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are present in the media serum supplement. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein L Purification Resin is also available as prepacked spin column and kit formats.
Alternative Protein L; Protein L resin

Image & description

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

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