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PurKine™ Protein AT Resin 4FF

PurKine™ Protein AT Resin 4FF

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Specification

Product name PurKine™ Protein AT Resin 4FF
Applications notes PurKine™ Protein AT Resin 4FF has been immobilized for the capture of monoclonal antibodies at process scale. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A(Alkaline tolerate)has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein A(Alkaline tolerate) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.
Alternative Protein AT; Protein AT resin; Protein Alkaline Tolerate

Product Properties

Formulation Liquid solution with 50% slurry in 20% ethanol.
Features & Benefits • High capacity—More than 40mg human lgG per mL of resin.
• High performance—Alkali-tolerant rProtein A ligand withstands rigorous Clean-in-place(CIP) and sanitization procedures with 0.1 to 0.5 M NaOH. The medium load is almost unchanged after hundreds of CIP. Oriented ligand coupling for optimal binding capacity.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.
Storage buffer Liquid in PBS buffer containing 20% ethanol.
Storage instructions Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PurKine™ Protein AT Purification system is effective for affinity purification of monoclonal antibodies at process scale. Protein At is an alkali-resistant protein A (Alkaline tolerate) based on the native protein A though biotechnology mutation. This ligand provides greater stability than conventional protein A, and cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein AT Purification Resin is also available as prepacked spin column and kit formats.
Alternative Protein AT; Protein AT resin; Protein Alkaline Tolerate

Image & description

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

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