- Product name
PurKine™ Protein AminoBind Resin 4FF
- Application notes
PurKine™ Protein AminoBind Resin 4FF is a pre-activated agarose matrix that increases the choice of coupling chemistries available.It is suitable for coupling amino-containing smaller proteins and peptides.AminoBind Resin 4FF is crosslinked, 4% beaded agarose resin that contains N-hydroxysuccinimide (NHS) functional groups.Its high flow properties make it excellent for scaling-up.
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.
Liquid solution with 50% slurry in 100% isopropanol.
- Features & Benefits
• High capacity—More than 10mg human lgG per mL of resin.
• High performance—NHS coupling forms a chemically stable amide bone with ligands containing primary amino groups.AminoBind Resin 4FF provides a spacer arm and is therefore particularly suitable for immobilizing small protein and peptide ligands.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 250cm/hour.
- Storage buffer
Liquid in 100% isopropanol.
- Storage instructions
Stable for one year at 2-8°C from date of shipment. Do not freeze.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
PurKine™ Protein AminoBind Resin 4FF is crosslinked, 4% beaded agarose resin that contains N-hydroxysuccinimide (NHS) functional groups. NHS coupling forms a chemically stableamide bone with ligands containing primary amino groups. PurKine™ Protein AminoBind Resin 4FF can be used to prepare affinity purification procedures which can isolate specific substances from complex mixtures, often achieving very high purity in a single step. PurKine™ Protein AminoBind Resin 4FF fulfills industrial demands for security of supply and robust performance.
- Alternative names
NHS-Activated Resin; NHS
Most popular with customers
PurKine™ Protein AminoBind Resin 4FF
Click to download booklet for more details
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.