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PurKine™ His-Tag Ni-Super Resin

PurKine™ His-Tag Ni-Super Resin

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Specification

Product name PurKine™ His-Tag Ni-Super Resin
Applications notes PurKine™ His-Tag Ni-Super Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Super replacement to NTA has been coupled, and then been charged with nickel ions (Ni2+) closely. This featured chelating group have more stable binding with Nii2+ than NTA, thus featured more resistance to various chemicals including reducing, chelating agents. Tests confirm that no decrease in performance occurs after at least five repeated uses. This His-Tag Ni-Super Resin is suitable for purification of His-tag proteins from samples that can cause Ni stripping from the medium, e.g. secreted proteins in solution containing chelators.
Alternative Ni-NTA; His resin; Ni-NTA resin; His tag resin

Product Properties

Formulation Liquid solution
Features & Benefits • High capacity—More than 10 mg of 6xHis-tagged protein per milliliter of resin.
• High toleration—More tolerable to various chemicals including reducing, chelating agents.The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA.
• Versatile—For purification under native or denaturing conditions.
• Cost-saving—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.• Easy Cleaning—Directly use NaOH to cleaning-in-place.
Storage buffer Liquid in PBS buffer containing 20% ethanol.
Storage instructions Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative Ni-NTA; His resin; Ni-NTA resin; His tag resin

Image & description

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

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