PurKine™ His-Tag Ni-IDA Resin

PurKine™ His-Tag Ni-IDA Resin has the advantages of higher binding capacity and cost-effective than Ni-NTA Beads , but it's only suitable for purification of soluble proteins under native conditions.

Product Information

  • Product name

    PurKine™ His-Tag Ni-IDA Resin

  • Application notes

    PurKine™ His-Tag Ni-IDA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Iminodiacetic Acid (IDA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-IDA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Product Properties

  • Formulation

    Liquid solution with 50% slurry in 20% ethanol.

  • Features & Benefits

    • High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin.
    • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
    • Flexible—available in multiple formats including bulk resin, spin columns.

  • Storage buffer

    Liquid in PBS buffer containing 20% ethanol.

  • Storage instructions

    Stable for one year at 2-8°C from date of shipment. Do not freeze.

  • Shipping

    Blue ice

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.

  • Alternative names

    Ni-IDA; His resin; Ni-IDA resin

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PurKine™ His-Tag Ni-IDA Resin
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: BMR20000

  • Size
  • Price
  • 5ml $80
  • 10ml $128
  • 50ml $512
  • 100ml $820

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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