PurKine™ His-Tag IMAC-NTA Resin 6FF

Product Information

  • Product name

    PurKine™ His-Tag IMAC-NTA Resin 6FF

  • Application notes

    PurKine™ His-Tag IMAC-NTA Resin 6FF effectively purifies high levels of overexpressed His-tagged fusion proteins .The Resin consists of 90μm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled, while the chelating group has not been charged, which is supplied as a metal-free gel allowing researchers to add a chelating metal of choice (e.g. Cu2+,Zn2+,Ni2+,or Co2+). Tests confirm that performance equals or exceeds popular IMAC resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    • High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin.
    • Versatile—For purification under native or denaturing conditions.
    • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
    • Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
    • Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour.

  • Storage buffer

    Liquid in PBS buffer containing 20% ethanol.

  • Storage instructions

    Stable for one year at 2-8°C from date of shipment. Do not freeze.

  • Shipping

    Blue ice

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.

  • Alternative names

    Ni-NTA; His resin; Ni-NTA resin; His tag resin

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PurKine™ His-Tag IMAC-NTA Resin 6FF
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: BMR20036

  • Size
  • Price
  • 5ml $132
  • 10ml $212
  • 50ml $844
  • 100ml $1350

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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