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PurKine™ GST-Tag Glutathione Resin 4FF

PurKine™ GST-Tag Glutathione Resin 4FF

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Specification

Product name PurKine™ GST-Tag Glutathione Resin 4FF
Applications notes PurKine™ GST-Tag Glutathione Resin 4FF effectively purifies high levels of overexpressed GST-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which reduced glutathione has been coupled. The glutathione ligand is coupled via a 12-atom linker.Tests confirm that performance equals or exceeds popular GST-Tag Purification Resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
Alternative Glutathione; GST resin; Glutathione resin; GST tag resin

Product Properties

Formulation Liquid solution with 50% slurry in 20% ethanol.
Features & Benefits • High capacity—More than 10 mg of GST-tagged protein per milliliter of resin.
• High performance—Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Easy to use— Pre-formulated buffers available for kit formats.
• Robust—highly crosslinked beads tolerate linear flow rates up to 450cm/hour.
Storage buffer Liquid in PBS buffer containing 20% ethanol.
Storage instructions Stable for one year at 2-8°C from date of shipment. Do not freeze.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PurKine™ GST-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ GST-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative Glutathione; GST resin; Glutathione resin; GST tag resin

Image & description

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.

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