|Product name||PurKine™ Benzamidine Resin 4FF|
|Applications notes||PurKine™ Benzamidine Resin 4FF has been widely used in removal and/or affinity purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein from serum, monoclonal cell supernatants and bacterial lysates. The Resin consists of 90μm beads of cross-linked 4% agarose, to which aminobenzamidine has been coupled. Tests confirm that performance equals or exceeds popular aminobenzamidine resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.|
|Formulation||Liquid solution with 50% slurry in 0.05 M Sodium Acetate contain 20% Ethanol,PH 4.0|
|Features & Benefits||• High capacity—More than 20mg trypsin per mL of resin.
• High performance—Also effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.
|Storage buffer||Liquid in 0.05 M Sodium Acetate containing 20% ethanol.|
|Storage instructions||Stable for one year at 2-8°C from date of shipment. Do not freeze.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||PurKine™ Benzamidine Resin 4FF is effective for removal and/or affinity purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein from serum, monoclonal cell supernatants and bacterial lysates. The Resin consists of 90μm beads of cross-linked 4% agarose, to which aminobenzamidine has been coupled. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Benzamidine Resin 4FF is also available as prepacked spin column and kit formats.|
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.