|Product name||PurKine™ Anti-DDDDK Tag Resin 4FF|
|Applications notes||PurKine™ Anti-DDDDK Tag Resin 4FF has been widely used in affinity purification of Flag fusion proteins.The Resin consists of 90μm beads of cross-linked 4% agarose, to which mouse monoclonal antibody against DYKDDDDK tag has been coupled. Tests confirm that performance equals or exceeds popular Anti-DDDDK Tag resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.In addition, its high flow properties make it excellent for scaling-up.|
|Formulation||Liquid solution with 50% slurry in TBS contain 0.02% sodium azide|
|Features & Benefits||• High capacity—More than 1mg DYKDDDDK-tagged protein per mL of resin.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 300cm/hour.
|Storage buffer||Liquid in TBS contain 0.02% sodium azide|
|Storage instructions||Stable for one year at 2-8°C from date of shipment. Do not freeze.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||PurKine™ Anti-DDDDK Resin 4FF has been designed for affinity purification of DDDDK fusion proteins. The Resin consists of 90μm beads of cross-linked 4% agarose, to which mouse monoclonal antibody against DYKDDDDK tag has been coupled. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Anti-DDDDK Resin 4FF is also available as prepacked spin column and kit formats.|
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.