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PSA Polyclonal Antibody

PSA Polyclonal Antibody

Views(47) Publications(0) Catalog no(ABP53282)
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Specification

Product name PSA Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human PSA
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative KLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK3 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
Gene ID 354
Alternative KLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Others PSA Polyclonal Antibody detects endogenous levels of PSA protein.
Accession P07288
Observed Band(KD) 29

Image & description

Fig.1. Western Blot analysis of NIH-3T3 cells using PSA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of NIH-3T3 cells using PSA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100.

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