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PKC ζ (phospho Thr560) Polyclonal Antibody

PKC ζ (phospho Thr560) Polyclonal Antibody

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Specification

Product name PKC ζ (phospho Thr560) Polyclonal Antibody
Immunogen Synthesized peptide derived from human PKC ζ around the phosphorylation site of T560
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 5590
Alternative PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
Others Phospho-PKC ζ (T560) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T560.
Accession Q05513
Observed Band(KD) 80

Image & description

Fig. Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000.

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