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PKC α (phospho Tyr657) Polyclonal Antibody

PKC α (phospho Tyr657) Polyclonal Antibody

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Specification

Product name PKC α (phospho Tyr657) Polyclonal Antibody
Immunogen Synthesized peptide derived from human PKC α around the phosphorylation site of Y657
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCA (protein kinase C alpha) is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Gene ID 5578
Alternative PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
Others Phospho-PKC α (Y657) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at Y657.
Accession P17252
Observed Band(KD) 80

Image & description

Fig. Western Blot analysis of 293 cells using Phospho-PKC α (Y657) Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of 293 cells using Phospho-PKC α (Y657) Polyclonal Antibody diluted at 1:1000.

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