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PI3 Kinase P85α Monoclonal Antibody

PI3 Kinase P85α Monoclonal Antibody

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Specification

Product name PI3 Kinase P85α Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase s

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
Gene ID 5295
Alternative PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase s
Accession P27986
Observed Band(KD) 85

Image & description

Fig.1. Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85α Mouse mAb diluted at 1:2000.

Fig.1. Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85α Mouse mAb diluted at 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using PI3 Kinase P85 α Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Rat Liver Tissue using PI3 Kinase P85 α Mouse mAb diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse BrainTissue using PI3 Kinase P85 α Mouse mAb diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse BrainTissue using PI3 Kinase P85 α Mouse mAb diluted at 1:200.

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