Login Register
English
0

Cart

$ 0

PI 3-kinase p85α Polyclonal Antibody

PI 3-kinase p85α Polyclonal Antibody

Views(663) Publications(0) Catalog no(ABP57236)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name PI 3-kinase p85α Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Human
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:200-1:500).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 (phosphoinositide-3-kinase regulatory subunit 1) encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.
Gene ID 5295
Others The antibody detects endogenous PI 3-kinase p85α protein.
Accession P27986
Observed Band(KD) 85

Image & description

Fig.1. Western blot analysis of 1) HepG2,  2) 293T using PI 3-kinase p85α Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of 1) HepG2, 2) 293T using PI 3-kinase p85α Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using PI 3-kinase p85α Polyclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using PI 3-kinase p85α Polyclonal Antibody.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “PI 3-kinase p85α Polyclonal Antibody”