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Phospho-Smad3 (S425) Mouse Monoclonal Antibody (1D9)

Phospho-Smad3 (S425) Mouse Monoclonal Antibody (1D9)

Views(63) Publications(0) Catalog no(ABM40339)
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Specification

Product name Phospho-Smad3 (S425) Mouse Monoclonal Antibody (1D9)
Immunogen Synthetic Peptide of Phospho-Smad3 (S425)
Host Mouse
Reactivity Human, Mouse, Rat
Applications IHC-P
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:50-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by SMAD3 (SMAD family member 3) belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Gene ID 4088
Alternative Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Others The antibody detects endogenous Phospho-Smad3(S425) protein.
Accession P84022
Observed Band(KD) 52

Image & description

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (S425) Mouse mAb diluted at 1:200.

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (S425) Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Phospho-Smad3 (S425) Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Phospho-Smad3 (S425) Mouse mAb diluted at 1:200.

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