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Pan Methyl Lysine Polyclonal Antibody

Pan Methyl Lysine Polyclonal Antibody

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Specification

Product name Pan Methyl Lysine Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity All
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative Lysine

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. In eukaryotes, histone H3 trimethylated at lysine 4 (H3K4me3) is associated with active chromatin and gene expression.
Alternative Lysine
Others The antibody detects endogenous protein Methylated Lysine.

Image & description

Fig. Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain tissue, diluted at 1:2000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain tissue, diluted at 1:2000. Secondary antibody was diluted at 1:20000.

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