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NOS2 Polyclonal Antibody

NOS2 Polyclonal Antibody

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Specification

Product name NOS2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human NOS2 at AA range: 1070-1150
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NOS2 encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Gene ID 4843
Alternative NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Others NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.
Accession P35228
Observed Band(KD) 130

Image & description

Fig.1. Western Blot analysis of various cells using NOS2 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using NOS2 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of hela cells using NOS2 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of hela cells using NOS2 Polyclonal Antibody diluted at 1:500.

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