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NFκB-p100 Polyclonal Antibody

NFκB-p100 Polyclonal Antibody

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Specification

Product name NFκB-p100 Polyclonal Antibody
Immunogen Synthesized peptide derived from human NFκB-p100 around the non-phosphorylation site of S865
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 ug/mg lysate), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NFKB2 encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. Nuclear factor kappa B subunit 2 encoded by NFKB2 can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for NFKB2 on chromosome 18. Alternative splicing results in multiple transcript variants.
Gene ID 4791
Alternative NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Others NFκB-p100 Polyclonal Antibody detects endogenous levels of NFκB-p100 protein.
Accession Q00653

Image & description

Fig.1. Western Blot analysis of various cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of K562 cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of K562 cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000.

Fig.3. Western blot analysis of KB, K562,  hela and 293T lysates using NFκB-p100 antibody. Antibody was diluted at 1:1000.

Fig.3. Western blot analysis of KB, K562, hela and 293T lysates using NFκB-p100 antibody. Antibody was diluted at 1:1000.

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