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Myf-5 Polyclonal Antibody

Myf-5 Polyclonal Antibody

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Specification

Product name Myf-5 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Myf-5 at AA range: 30-110
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MYF5 is a Protein Coding gene. Myogenic Factor 5 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. Diseases associated with MYF5 include skeletal muscle cancer and botryoid rhabdomyosarcoma. Among its related pathways are Developmental Biology and CDO in myogenesis. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1.
Gene ID 4617
Alternative MYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2
Others Myf-5 Polyclonal Antibody detects endogenous levels of Myf-5 protein.
Accession P13349
Observed Band(KD) 28

Image & description

Fig.1. Western Blot analysis of various cells using Myf-5 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Myf-5 Polyclonal Antibody.

Fig.2. Western Blot analysis of HepG2 cells using Myf-5 Polyclonal Antibody.

Fig.2. Western Blot analysis of HepG2 cells using Myf-5 Polyclonal Antibody.

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