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Mouse Zinc transporter 7 (SLC30A7) ELISA Kit

Mouse Zinc transporter 7 (SLC30A7) ELISA Kit

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Specification

Product name Mouse Zinc transporter 7 (SLC30A7) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Zinc transporter 7 (SLC30A7) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A7 in samples. An antibody specific for SLC30A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC30A7; DKFZp686M0368; ZNT7; ZnT-7; ZnTL2; zinc transporter ZnT-7; zinc transporter like 2

Product Properties

Kit components • Mouse Zinc transporter 7 microplate
• Mouse Zinc transporter 7 standard
• Mouse Zinc transporter 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Zinc transporter 7 (SLC30A7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC30A7. No significant cross-reactivity or interference between Mouse SLC30A7 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc.The deduced proteins share 96% amino acid identity. Mouse Znt7 contains 6 transmembrane domains, a histidine-rich loop between transmembrane domains 4 and 5, cytoplasmic N and C termini, several phosphorylation sites, and an N-glycosylation site. Northern blot analysis of mouse tissues detected highest expression in liver, spleen, and small intestine. In rat epithelial and human fibroblast cell lines, ZNT7 clustered at the perinuclear region and showed punctate staining in the cytoplasm, indicative of cytoplasmic vesicle localization.
Gene ID 66500
Alternative SLC30A7; DKFZp686M0368; ZNT7; ZnT-7; ZnTL2; zinc transporter ZnT-7; zinc transporter like 2
Accession Q9JKN1

Image & description

Fig.1. Mouse Zinc transporter 7 (SLC30A7) Standard Curve.

Fig.1. Mouse Zinc transporter 7 (SLC30A7) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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