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Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit

Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit

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Specification

Product name Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit employs a two-site sandwich ELISA to quantitate LSM5 in samples. An antibody specific for LSM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLSM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LSM5; FLJ12710; YER146W; LSM5 homolog; U6 small nuclear RNA associated; OTTHUMP00000202663; OTTHUMP00000202664; OTTHUMP00000202667; U6 snRNA-associated Sm-like protein

Product Properties

Kit components • Mouse U6 snRNA-associated Sm-like protein LSm5 microplate
• Mouse U6 snRNA-associated Sm-like protein LSm5 standard
• Mouse U6 snRNA-associated Sm-like protein LSm5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LSM5. No significant cross-reactivity or interference between Mouse LSM5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family . Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.Disruption of genes encoding Sm-like proteins directly associated with the U6 snRNA (Lsm2-8) generated variable phenotypes. Lsm2, Lsm3, Lsm4, and Lsm8 are essential for vegetative growth. Lsm5, Lsm6, and Lsm7 are not essential for growth; however, their disruptions lead to slow growth especially at elevated temperature. The levels of the U6 snRNA were strongly reduced in the strains harboring the Lsm5, Lsm6, and Lsm7 disruptions.
Gene ID 66373
Alternative LSM5; FLJ12710; YER146W; LSM5 homolog; U6 small nuclear RNA associated; OTTHUMP00000202663; OTTHUMP00000202664; OTTHUMP00000202667; U6 snRNA-associated Sm-like protein
Accession P62322

Image & description

Fig.1. Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) Standard Curve.

Fig.1. Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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