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Mouse Transcription factor Maf (MAF) ELISA Kit

Mouse Transcription factor Maf (MAF) ELISA Kit

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Specification

Product name Mouse Transcription factor Maf (MAF) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Transcription factor Maf (MAF) ELISA Kit employs a two-site sandwich ELISA to quantitate MAF in samples. An antibody specific for MAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAF; MGC71685; c-MAF; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; T lymphocyte c-maf long form; c-maf proto-oncogene; v-maf musculoaponeurotic fibrosarcoma oncogene homolog

Product Properties

Kit components • Mouse Transcription factor Maf microplate
• Mouse Transcription factor Maf standard
• Mouse Transcription factor Maf detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Transcription factor Maf (MAF) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAF. No significant cross-reactivity or interference between Mouse MAF and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by MAF is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in MAF are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for MAF.
Gene ID 17132
Alternative MAF; MGC71685; c-MAF; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; T lymphocyte c-maf long form; c-maf proto-oncogene; v-maf musculoaponeurotic fibrosarcoma oncogene homolog
Accession P54843

Image & description

Fig.1. Mouse Transcription factor Maf (MAF) Standard Curve.

Fig.1. Mouse Transcription factor Maf (MAF) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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