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Mouse TNF-alpha protein

Product Information

  • Product name

    Mouse TNF-alpha protein

  • Sequence

    Amino acid sequence derived from Mouse TNF-alpha isoform (P06804) (Leu80-Leu235) was expressed.

  • Activity

    Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.

  • Protein length

    The recombinant Mouse TNF-alpha protein consists of 157 amino acids and has a predicted molecular mass of 17 kDa.

  • Preparation method

    E. coli

  • Purity

    >98 % as determined by SDS-PAGE

Fig. SDS-PAGE analysis of Mouse TNF-alpha protein.

Product Properties

  • Formulation

    Lyophilized from sterile PBS, pH 7.4.

  • Features & Benefits

    Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

  • Molecular weight

    17 kDa

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TNF-alpha protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized Mouse TNF-alpha protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

  • Gene ID

    21926

  • Alternative names

    TNFSF2; DIF; Necrosin; Cytotoxin

  • Accession

    P06804

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Mouse TNF-alpha protein
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP1113

  • Size
  • Price
  • 100 μg $350
  • 5 µg $48
  • 20 µg $120
  • 1 mg $2500

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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