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Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit

Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit

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Specification

Product name Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHB in samples. An antibody specific for SDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDHB; FLJ92337; IP; PGL4; SDH; SDH1; SDH2; SDHIP;

Product Properties

Kit components • Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial microplate
• Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial standard
• Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHB. No significant cross-reactivity or interference between Mouse SDHB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Complex II in mitochondria, of which succinate dehydrogenase (EC 1.3.99.1) is a component, has 4 subunits. In order of decreasing molecular mass, they are the flavoprotein (SDHA), the iron sulfur protein (SDHB), and the 2 integral membrane proteins, SDHC and SDHD. Kita et al. (1990) cloned and sequenced the iron sulfur protein subunit. A clone was isolated from a human liver cDNA library. The open reading frame encodes a 252-amino acid protein. The amino acid sequence showed approximately 94% homology with that of bovine heart. Au et al. (1995) described the complete genomic clone for the gene encoding the iron sulfur protein subunit.
Gene ID 67680
Alternative SDHB; FLJ92337; IP; PGL4; SDH; SDH1; SDH2; SDHIP;
Accession Q9CQA3

Image & description

Fig.1. Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) Standard Curve.

Fig.1. Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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