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Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit

Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit

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Specification

Product name Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHA in samples. An antibody specific for SDHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDHA; FP; SDH1; SDH2; SDHF; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex; subunit A; flavoprotein

Product Properties

Kit components • Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial microplate
• Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial standard
• Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHA. No significant cross-reactivity or interference between Mouse SDHA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SDHA encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Hirawake et al. (1994) isolated a cDNA corresponding to the flavoprotein subunit of SDH from a human liver cDNA library. The deduced 621-amino acid protein has a molecular mass of approximately 68 kD and shows high homology to the bovine heart flavoprotein subunit.
Gene ID 66945
Alternative SDHA; FP; SDH1; SDH2; SDHF; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex; subunit A; flavoprotein
Accession Q8K2B3

Image & description

Fig.1. Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) Standard Curve.

Fig.1. Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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