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Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) ELISA Kit

Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) ELISA Kit

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Specification

Product name Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR5 in samples. An antibody specific for S1PR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative S1PR5; EDG8; Edg-8; S1P5; SPPR-1; SPPR-2; endothelial differentiation; sphingolipid G-protein-coupled receptor; 8; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8

Product Properties

Kit components • Mouse Sphingosine 1-phosphate receptor 5 microplate
• Mouse Sphingosine 1-phosphate receptor 5 standard
• Mouse Sphingosine 1-phosphate receptor 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S1PR5. No significant cross-reactivity or interference between Mouse S1PR5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background According to these guidelines, a receptor is to be named with the abbreviation of the natural agonist with the highest potency, followed by a subscripted arabic number. The conceptualized 400-amino acid rat Edg8 protein shares 42 to 49% amino acid identity with the human S1P receptors EDG1, EDG3, and EDG5. Binding analysis confirmed that expression of Edg8 leads to high-affinity binding for labeled S1P.Widely expressed in the brain, most prominently in the corpus callosum, which is predominantly white matter. Detected in spleen, peripheral blood leukocytes, placenta, lung, aorta, and fetal spleen. Low-level signal detected in many tissue extracts. Isoform 1 is predominently expressed in peripheral tissues, whereas isoform 2 is expressed in brain, spleen and peripheral blood leukocytes.
Gene ID 94226
Alternative S1PR5; EDG8; Edg-8; S1P5; SPPR-1; SPPR-2; endothelial differentiation; sphingolipid G-protein-coupled receptor; 8; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8
Accession Q91X56

Image & description

Fig.1. Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) Standard Curve.

Fig.1. Mouse Sphingosine 1-phosphate receptor 5 (S1PR5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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