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Mouse Sperm protamine P1 (PRM1) ELISA Kit

Mouse Sperm protamine P1 (PRM1) ELISA Kit

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Specification

Product name Mouse Sperm protamine P1 (PRM1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Sperm protamine P1 (PRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM1 in samples. An antibody specific for PRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRM1; CT94.1; P1; cancer/testis antigen family 94; member 1; sperm protamine P1; testis specific protamine 1

Product Properties

Kit components • Mouse Sperm protamine P1 microplate
• Mouse Sperm protamine P1 standard
• Mouse Sperm protamine P1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Sperm protamine P1 (PRM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRM1. No significant cross-reactivity or interference between Mouse PRM1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protamines are small, arginine-rich, nuclear proteins that replace histones late in the haploid phase of spermatogenesis and are believed essential for sperm head condensation and DNA stabilization. Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine. The 2 human protamines are denoted P1 and P2 (PRM2). PRM1 and PRM2 have a single intron, consisting of 91 and 163 bp, respectively. Both genes were found to contain typical TATAA and CAAT boxes at conventional distances from the transcription start points which, by use of primary extension experiments, were assigned to nucleotides -91 and -110 for PRM1 and PRM2, respectively.
Gene ID 19118
Alternative PRM1; CT94.1; P1; cancer/testis antigen family 94; member 1; sperm protamine P1; testis specific protamine 1
Accession P02319

Image & description

Fig.1. Mouse Sperm protamine P1 (PRM1) Standard Curve.

Fig.1. Mouse Sperm protamine P1 (PRM1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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