Login Register
English
0

Cart

$ 0

Mouse Spartin (SPG20) ELISA Kit

Mouse Spartin (SPG20) ELISA Kit

Views(118) Publications(0) Catalog no(KTE70386)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Mouse Spartin (SPG20) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Spartin (SPG20) ELISA Kit employs a two-site sandwich ELISA to quantitate SPG20 in samples. An antibody specific for SPG20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPG20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPG20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPG20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPG20; KIAA0610; SPARTIN; TAHCCP1; spartin; trans-activated by hepatitis C virus core protein 1

Product Properties

Kit components • Mouse Spartin microplate
• Mouse Spartin standard
• Mouse Spartin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Spartin (SPG20) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPG20. No significant cross-reactivity or interference between Mouse SPG20 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SPG20 encodes a protein that contains a MIT (Microtubule Interacting and Trafficking molecule) domain. This protein may be involved in endosomal trafficking, microtubule dynamics, or both functions. Frameshift mutations associated with SPG20 cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). Troyer syndrome (SPG20) is a complicated type of hereditary spastic paraplegias (HSPs). HSP is a category of neurological disorder characterized by spasticity and muscle weakness in the lower limbs.
Gene ID 229285
Alternative SPG20; KIAA0610; SPARTIN; TAHCCP1; spartin; trans-activated by hepatitis C virus core protein 1
Accession Q8R1X6

Image & description

Fig.1. Mouse Spartin (SPG20) Standard Curve.

Fig.1. Mouse Spartin (SPG20) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Mouse Spartin (SPG20) ELISA Kit”