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Mouse Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit

Mouse Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit

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Specification

Product name Mouse Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1 in samples. An antibody specific for MYH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYH1; MGC133384; MYHSA1; MYHa; MyHC-2X/D; myosin heavy chainx/d; myosin; heavy polypeptide 1; skeletal muscle; adult

Product Properties

Kit components • Mouse Soluble myosin heavy chain 1 microplate
• Mouse Soluble myosin heavy chain 1 standard
• Mouse Soluble myosin heavy chain 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYH1. No significant cross-reactivity or interference between Mouse MYH1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Gene ID 17879
Alternative MYH1; MGC133384; MYHSA1; MYHa; MyHC-2X/D; myosin heavy chainx/d; myosin; heavy polypeptide 1; skeletal muscle; adult
Accession Q5SX40

Image & description

Fig.1. Mouse Soluble myosin heavy chain 1 (sMHC-1) Standard Curve.

Fig.1. Mouse Soluble myosin heavy chain 1 (sMHC-1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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